From bottleneck to breakthrough: Rethinking downstream processing with single-use separation

As cell densities and titers continue to rise, harvest and clarification are increasingly becoming the limiting operations during scale-up and commercial manufacturing. Upstream intensification has moved quickly - downstream technology has not always kept pace.

Join Aysha Kamran, Single-Use Separation Specialist, and Ivan Gitardi, Business Development Manager for Food & Pharma at Alfa Laval, as they explore the emerging gap between upstream intensification and conventional clarification technologies. Drawing on customer experiences, published industry studies and real application work, they will discuss how CultureOne™ single-use disc-stack centrifugation enables robust harvest of highly intensified cultures at approximately 30 to 200 million CHO cells/mL (6–40% PCV) - while maintaining high product recovery, scalable performance and gentle product handling.

Key learnings

• Where conventional clarification breaks down as cultures intensify, and why the gap is widening
• How single-use disc-stack centrifugation handles high-density harvest at 30–200 million CHO cells/mL
• The impact of single-use separation on product recovery, yield and gentle product handling
• How eliminating cleaning validation reduces changeover time and contamination risk in multiproduct facilities
• Real customer case studies demonstrating scalable performance from clinical through to commercial production
• Practical considerations for integrating single-use separation into existing downstream processes

Is this webinar for you?

Whether you are involved in process development, downstream processing, biologics manufacturing, technology transfer, scale-up or quality assurance, this webinar will give you actionable insight into how single-use separation can remove bottlenecks from your harvest and clarification steps.

The session runs for 30 minutes including a live Q&A, and is available at two times on Thursday 12 November - 11:00 CET and 16:00 CET. Both sessions cover identical content. If you are unable to attend either, register anyway and we will send you the recording.